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ice cold facs buffer  (Thermo Fisher)


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    Thermo Fisher ice cold facs buffer
    Ice Cold Facs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ice+cold+facs+buffer/Phosphate/pmc12996326-440-21-32
    Average 99 stars, based on 1 article reviews
    ice cold facs buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    FACS:

    Article Title: Antigenic imprinting dominates humoral responses to new variants of SARS-CoV-2 in a hamster model of COVID-19
    Article Snippet: Total anti S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK cells stably transfected with spike protein from Wuhan-Hu-1 strain (Mono8 cells). .. Mono8 cells (105 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Corning, New York, USA) and blocked from 20 min in ice-cold FACS buffer (2% fetal calf serum in Dulbecco’s Phosphate Buffered Saline (Gibco) supplemented with 2.5 mM EDTA (Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA)). ..

    Article Title: In vitro assessment of ATP-binding cassette transporters and their functional genetic polymorphisms on fluoroquinolone accumulation in human embryonic kidney 293 recombinant cell lines.
    Article Snippet: Fluoroquinolone tissue distribution and cellular accumulation are hindered by efflux transporters, including ATP-binding cassette subfamilyBmember1 (ABCB1), ATP-binding cassette subfamilyGmember2 (ABCG2), and ATP-binding cassette subfamily C member 4 (ABCC4).. Genetic polymorphisms (single-nucleotide polymorphisms) can impact transporter activity, leading to interindividual variability in the systemic and cellular pharmacokinetics of their substrates.. This study assesses the impact of these transporters onmoxifloxacin and ciprofloxacin (CIP) cellular accumulation invitro, and the effect of common single-nucleotide polymorphisms in ABCB1 [c.1199G>A (rs2229109); common haplotype c.1236C>T (rs1128503), c.2677G>T/A (rs2032582), and c.3435C>T (rs1045642)] and ABCG2 [c.421C>A (rs2231142)].

    Article Title: Common cold embecovirus imprinting primes broadly neutralizing antibody responses to SARS-CoV-2 S2
    Article Snippet: .. After 30 minutes of incubation on ice, the stained B cells were washed once with ice-cold FACS buffer (1X PBS supplemented with 0.2% bovine serum albumin [BSA] and 2 mM Pierce Biotin), centrifuged at 300 g for 10 minutes at 4°C, and then stained for 10 minutes with Live/Dead BV510 (Thermo Fisher Scientific). .. Cells of interest were identified as viable CD3−CD19+Antigen-PE+Antigen-APC and subsequently bulk-sorted using the MACSQuant Tyto cartridge sorting platform (Miltenyi).

    Article Title: Antigenic Imprinting Dominates Humoral Responses to New Variants of SARS-CoV-2 in a Hamster Model of COVID-19
    Article Snippet: Total anti S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK293 cells stably transduced with spike protein from Wuhan-Hu-1 strain (Mono8 cells). .. Mono8 cells (10 5 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Corning, NY, USA) and blocked for 20 min in ice-cold FACS buffer (Dulbecco’s Phosphate Buffered Saline (DPBS; Gibco) supplemented with 2% FCS (HyClone TM ) and 2.5 mM ethylenediaminetetraacetic acid (EDTA; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: COVID-19-related inflammation of the placenta impedes fetal development in pregnant hamsters.
    Article Snippet: Total anti-S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK cells stably expressing the S protein from the Wuhan-Hu-1 strain (hereafter, Mono8 cells). .. Mono8 cells (105 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Cat#3799) and blocked for 20 min in ice-cold FACS buffer (2% fetal calf serum in Dulbecco’s Phosphate Buffered Saline (Gibco) supplemented with 2.5 mM EDTA (Invitrogen, Cat#15-575-020). ..

    Article Title: Evidence of antigenic drift in the fusion machinery core of SARS-CoV-2 spike
    Article Snippet: Then, cells were transfected with 2 μg of plasmid encoding WT S or the indicated mutant using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol. .. At 20 h posttransfection, cells were harvested and resuspended in ice-cold FACS buffer [2% v/v FBS, 50 mM EDTA in DMEM supplemented with high glucose, L-glutamine and HEPES, without phenol red (Gibco)]. .. Cells were incubated with 5 μg/mL of RBD antibody CC12.3 ( ) for 1 h at 4 °C with gentle rocking.

    Article Title: COVID-19-related inflammation of the placenta impedes fetal development in pregnant hamsters
    Article Snippet: Total anti-S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK cells stably expressing the S protein from the Wuhan-Hu-1 strain (hereafter, Mono8 cells). .. Mono8 cells (10 5 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Cat#3799) and blocked for 20 min in ice-cold FACS buffer (2% fetal calf serum in Dulbecco’s phosphate-buffered saline (Gibco) supplemented with 2.5 mM EDTA (Invitrogen, Cat#15-575-020). ..

    Saline:

    Article Title: Antigenic imprinting dominates humoral responses to new variants of SARS-CoV-2 in a hamster model of COVID-19
    Article Snippet: Total anti S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK cells stably transfected with spike protein from Wuhan-Hu-1 strain (Mono8 cells). .. Mono8 cells (105 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Corning, New York, USA) and blocked from 20 min in ice-cold FACS buffer (2% fetal calf serum in Dulbecco’s Phosphate Buffered Saline (Gibco) supplemented with 2.5 mM EDTA (Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA)). ..

    Article Title: Antigenic Imprinting Dominates Humoral Responses to New Variants of SARS-CoV-2 in a Hamster Model of COVID-19
    Article Snippet: Total anti S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK293 cells stably transduced with spike protein from Wuhan-Hu-1 strain (Mono8 cells). .. Mono8 cells (10 5 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Corning, NY, USA) and blocked for 20 min in ice-cold FACS buffer (Dulbecco’s Phosphate Buffered Saline (DPBS; Gibco) supplemented with 2% FCS (HyClone TM ) and 2.5 mM ethylenediaminetetraacetic acid (EDTA; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: COVID-19-related inflammation of the placenta impedes fetal development in pregnant hamsters.
    Article Snippet: Total anti-S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK cells stably expressing the S protein from the Wuhan-Hu-1 strain (hereafter, Mono8 cells). .. Mono8 cells (105 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Cat#3799) and blocked for 20 min in ice-cold FACS buffer (2% fetal calf serum in Dulbecco’s Phosphate Buffered Saline (Gibco) supplemented with 2.5 mM EDTA (Invitrogen, Cat#15-575-020). ..

    Article Title: COVID-19-related inflammation of the placenta impedes fetal development in pregnant hamsters
    Article Snippet: Total anti-S-protein IgG antibodies induced by vaccination were evaluated in serum samples by flow cytometry using HEK cells stably expressing the S protein from the Wuhan-Hu-1 strain (hereafter, Mono8 cells). .. Mono8 cells (10 5 cells/well) were plated in a U-bottom 96-well plate (Corning Inc., Cat#3799) and blocked for 20 min in ice-cold FACS buffer (2% fetal calf serum in Dulbecco’s phosphate-buffered saline (Gibco) supplemented with 2.5 mM EDTA (Invitrogen, Cat#15-575-020). ..

    Expressing:

    Article Title: In vitro assessment of ATP-binding cassette transporters and their functional genetic polymorphisms on fluoroquinolone accumulation in human embryonic kidney 293 recombinant cell lines.
    Article Snippet: Fluoroquinolone tissue distribution and cellular accumulation are hindered by efflux transporters, including ATP-binding cassette subfamilyBmember1 (ABCB1), ATP-binding cassette subfamilyGmember2 (ABCG2), and ATP-binding cassette subfamily C member 4 (ABCC4).. Genetic polymorphisms (single-nucleotide polymorphisms) can impact transporter activity, leading to interindividual variability in the systemic and cellular pharmacokinetics of their substrates.. This study assesses the impact of these transporters onmoxifloxacin and ciprofloxacin (CIP) cellular accumulation invitro, and the effect of common single-nucleotide polymorphisms in ABCB1 [c.1199G>A (rs2229109); common haplotype c.1236C>T (rs1128503), c.2677G>T/A (rs2032582), and c.3435C>T (rs1045642)] and ABCG2 [c.421C>A (rs2231142)].

    Flow Cytometry:

    Article Title: In vitro assessment of ATP-binding cassette transporters and their functional genetic polymorphisms on fluoroquinolone accumulation in human embryonic kidney 293 recombinant cell lines.
    Article Snippet: Fluoroquinolone tissue distribution and cellular accumulation are hindered by efflux transporters, including ATP-binding cassette subfamilyBmember1 (ABCB1), ATP-binding cassette subfamilyGmember2 (ABCG2), and ATP-binding cassette subfamily C member 4 (ABCC4).. Genetic polymorphisms (single-nucleotide polymorphisms) can impact transporter activity, leading to interindividual variability in the systemic and cellular pharmacokinetics of their substrates.. This study assesses the impact of these transporters onmoxifloxacin and ciprofloxacin (CIP) cellular accumulation invitro, and the effect of common single-nucleotide polymorphisms in ABCB1 [c.1199G>A (rs2229109); common haplotype c.1236C>T (rs1128503), c.2677G>T/A (rs2032582), and c.3435C>T (rs1045642)] and ABCG2 [c.421C>A (rs2231142)].

    Centrifugation:

    Article Title: In vitro assessment of ATP-binding cassette transporters and their functional genetic polymorphisms on fluoroquinolone accumulation in human embryonic kidney 293 recombinant cell lines.
    Article Snippet: Fluoroquinolone tissue distribution and cellular accumulation are hindered by efflux transporters, including ATP-binding cassette subfamilyBmember1 (ABCB1), ATP-binding cassette subfamilyGmember2 (ABCG2), and ATP-binding cassette subfamily C member 4 (ABCC4).. Genetic polymorphisms (single-nucleotide polymorphisms) can impact transporter activity, leading to interindividual variability in the systemic and cellular pharmacokinetics of their substrates.. This study assesses the impact of these transporters onmoxifloxacin and ciprofloxacin (CIP) cellular accumulation invitro, and the effect of common single-nucleotide polymorphisms in ABCB1 [c.1199G>A (rs2229109); common haplotype c.1236C>T (rs1128503), c.2677G>T/A (rs2032582), and c.3435C>T (rs1045642)] and ABCG2 [c.421C>A (rs2231142)].

    Incubation:

    Article Title: Common cold embecovirus imprinting primes broadly neutralizing antibody responses to SARS-CoV-2 S2
    Article Snippet: .. After 30 minutes of incubation on ice, the stained B cells were washed once with ice-cold FACS buffer (1X PBS supplemented with 0.2% bovine serum albumin [BSA] and 2 mM Pierce Biotin), centrifuged at 300 g for 10 minutes at 4°C, and then stained for 10 minutes with Live/Dead BV510 (Thermo Fisher Scientific). .. Cells of interest were identified as viable CD3−CD19+Antigen-PE+Antigen-APC and subsequently bulk-sorted using the MACSQuant Tyto cartridge sorting platform (Miltenyi).

    Staining:

    Article Title: Common cold embecovirus imprinting primes broadly neutralizing antibody responses to SARS-CoV-2 S2
    Article Snippet: .. After 30 minutes of incubation on ice, the stained B cells were washed once with ice-cold FACS buffer (1X PBS supplemented with 0.2% bovine serum albumin [BSA] and 2 mM Pierce Biotin), centrifuged at 300 g for 10 minutes at 4°C, and then stained for 10 minutes with Live/Dead BV510 (Thermo Fisher Scientific). .. Cells of interest were identified as viable CD3−CD19+Antigen-PE+Antigen-APC and subsequently bulk-sorted using the MACSQuant Tyto cartridge sorting platform (Miltenyi).



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    ice cold facs buffer  (Fisher Scientific)
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    Fisher Scientific ice cold facs buffer
    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
    Ice Cold Facs Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ice cold facs buffer  (Thermo Fisher)
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    ice cold filter sterilized facs buffer  (Thermo Fisher)
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    ice cold facs buffer  (Jackson Immuno)
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Journal: bioRxiv

    Article Title: A Single-Cell Atlas of the Mouse Dural Meninges Reveals Pervasive Sex Differences Across Cellular Compartments

    doi: 10.64898/2026.05.12.721894

    Figure Lengend Snippet: A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Article Snippet: The cell pellets were resuspended in ice-cold FACS buffer (0.1M PBS; 1mM EDTA (Fisher Scientific) and 1% BSA (Equitech-Bio; BAH70); pH 7.4).

    Techniques: Isolation, Expressing, Quantitative Proteomics